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Recombinant Zaire Ebola virus (GPΔmuc) (Sf9)

Recombinant Protein: Zaire Ebola virus (GPΔmuc) (Sf9), also known as Recombinant Ebola virus (EBOV) Glycoprotein minus Mucin Domain (rGPΔmuc), is supplied as purified protein.

EBOV rGPΔmuc is produced in Sf9 insect cells using baculovirus for expression.
Purified by FPLC

Formulation & Storage: Supplied in PBS (pH7.4) and supplemented with glycerol, arginine and glutamic acid. Store at -80°C.

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

EBOV rGPΔmuc is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$571.00

Recombinant Zaire Ebola virus GPΔTM (Makona strain)

Mature, recombinant, Ebola Virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM)

Pathogen Strain: Makona strain
Supplied as a purified protein.
Contains a His-tag
EBOV rGPΔTM is produced in stably-transfected Drosophila Schneider 2 (S2) cells
Purified by FPLC.

Formulation & Storage: Supplied in PBS (supplemented with glycerol, arginine and glutamic acid) Storage: 2-3 weeks at -20°C, -80°C long term
Notes & Usage Guidelines: ELISA: Assay-dependent dilution; WB: Assay-dependent dilution

Ebola virus GPΔTM is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$604.00

Recombinant Zaire Ebola virus GPΔTM (S2) – 100ug

Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM) is supplied as purified protein.

His-tagged
EBOV rGPΔTM is produced in stably transfected Drosophila Schneider 2 (S2) cells
Purified by FPLC

Formulation & Storage: Supplied in PBS and supplemented with glycerol, arginine and glutamic acid. Store at -20°C for 2-3 weeks; -80°C for long-term

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

Ebola virus rGPΔTM is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$604.00

Recombinant Zaire Ebola virus GPΔTM (S2) – 500ug

Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM) is supplied as purified protein.

His-tagged
EBOV rGPΔTM is produced in stably transfected Drosophila Schneider 2 (S2) cells
Purified by FPLC

Formulation & Storage: Supplied in PBS and supplemented with glycerol, arginine and glutamic acid. Store at -20°C for 2-3 weeks; -80°C for long-term

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

Ebola virus GPΔTM (S2) is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$2,415.00

Recombinant Zaire Ebola virus GPΔTM (Sf9) – 100ug

Mature, recombinant, Ebolavirus Glycoprotein minus the transmembrane domain (Ebola virus GPΔTM (Sf9)) is supplied as purified protein.

His-tagged
EBOV rGPΔTM is produced in Sf9 insect cells using baculovirus for expression
Purified by FPLC

Formulation & Storage: Supplied in PBS in frozen format with no preservatives added. Store at +4°C for 2-3 weeks; -80°C for long-term.

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

Ebola virus GPΔTM is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$604.00

Recombinant Zaire Ebola virus GPΔTM (Sf9) – 500ug

Mature, recombinant, Ebolavirus Glycoprotein minus the transmembrane domain (Ebola virus GPΔTM (Sf9)) is supplied as purified protein.

His-tagged
EBOV rGPΔTM is produced in Sf9 insect cells using baculovirus for expression
Purified by FPLC

Formulation & Storage: Supplied in PBS in frozen format with no preservatives added. Store at +4°C for 2-3 weeks; -80°C for long-term.

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

Ebola virus GPΔTM is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$2,415.00

Recombinant Zaire Ebola virus VP40 (EBOV VP40)

Recombinant, tag-free, purified Zaire Ebola Virus VP40 matrix protein (EBOV VP40) is supplied as purified protein.

Tag-free
EBOV VP40 is produced in E. coli
Purified by affinity chromatography, FPLC

Formulation & Storage: Supplied in HEPES buffer (pH 7.5) containing sodium chloride, 5% glycerol and 0.01% Triton-X. Store at -80°C.

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

EBOV VP40 is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$604.00

rVSV pseudotyped Marburg Virus GP (Angola Strain)

Recombinant Vesicular Stomatitis Virus pseudotyped MARV-Angola glycoprotein (rVSV pseudotyped MARVAngola GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Marburg virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.

Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C.
Notes & Usage Guidelines:
Recommended Dilution Range: 1: 800
Luciferase Units for Assays: 30,000

Please email products@integratedbiotherapeutics.com for lot specific information for ‘Titer’ and ‘Usage’.

Price:

$689.00

rVSV pseudotyped Sudan Virus GP (Boniface Strain)

Recombinant Vesicular Stomatitis Virus pseudotyped Sudan glycoprotein (rVSV pseudotyped SUDV GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV pseudotypes containing the envelope glycoprotein of Sudan virus. Since the infectivity of rVSV pseudotyped SUDV GP is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment. Infectivity and neutralization of infectivity can be measured by luciferase activity.

Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C.
Notes & Usage Guidelines:
Recommended Dilution Range: 1: 40-1:80
Luciferase Units for Assays: 20,000-40,000

Please email products@integratedbiotherapeutics.com for lot specific information for ‘Titer’ and ‘Usage’.

Price:

$689.00

rVSV pseudotyped Sudan Virus GP (Gulu Strain)

rVSV pseudotyped Sudan Virus GP (Gulu Strain) or Recombinant Vesicular Stomatitis Virus pseudotyped Sudan Virus Gulu glycoprotein (rVSV-SUDV-Gulu-GP)) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSVpseudotyped virus containing the envelope glycoprotein of Sudan virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.

Price:

$689.00

rVSV-pseudotyped Ebola virus GP (rVSV-EBOV-GP)

Recombinant Vesicular Stomatitis Virus (rVSV) pseudotyped with Ebola virus glycoprotein (rVSV-EBOV-GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Ebola virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.

Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C.
Notes & Usage Guidelines:
Recommended Dilution: 1:40
Luciferase Units for Assays: 30,000
Reporter: Luciferase

Price:

$689.00

S. aureus LukF-PV (His-tag, C-terminus)

Purified, His-tagged Staphylococcus aureus PantonValentine Leukocidin (PVL) F subunit (LukF-PV) is supplied as purified protein.

His-tagged
LukF-PV is produced in E. coli.
Purified by column chromatography

Formulation & Storage: Supplied in PBS and 15% Glycerol. Store at -20°C for 2-3 weeks, -80°C for long term.

Notes & Usage Guidelines: ELISA: N/A WB: Assay-dependent dilution

LukF-PV is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$394.00

S. aureus LukF-PV (tag free)

Purified, Tag-Free, Recombinant Staphylococcus aureus Panton-Valentine Leukocidin (PVL) F subunit (LukF-PV) is supplied as purified protein.

Tag-free
LukF-PV is produced in E. coli.
Purified by column chromatography

Formulation & Storage: Supplied in PBS containing sodium chloride and 5% glycerol. Store at -20°C for 23 weeks, -80°C for long term.

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

LukF-PV is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$459.00

S. aureus recombinant Leukocidin-D (tag free)

Purified Staphylococcus aureus recombinant leukocidin-D (rLukD). is supplied as purified protein.

Tag-free
rLukD is produced in E. coli.
Purified by FPLC

Formulation & Storage: Supplied in PBS as a liquid solution. Store at -20°C for 2-3 weeks, -80°C for long-term.

Notes & Usage Guidelines: ELISA: N/A WB: Assay-dependent dilution

rLukD is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$427.00

S. aureus Recombinant LukS-PV (tag free)

Purified, Recombinant, tag-free, Staphylococcus aureus Panton-Valentine Leukocidin (PVL) S subunit (LukS-PV) is supplied as purified protein.

Tag-free
LukS-PV is produced in E. coli.
Purified by column chromatography

Formulation & Storage: Supplied in PBS and 5% glycerol in liquid solution. Store at -20°C for 2-3 weeks, -80°C for long term

Notes & Usage Guidelines: ELISA: Assay-dependent dilution WB: Assay-dependent dilution

LukS-PV is intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$459.00

SARS-CoV-2 Indicator Cell Line for RNA Replication – GFP Reporter only

SARS-CoV-2 GFP reporter cell line using HEK293T (ACE2/TMPRSS2) cells

Price:

$6,500.00

SARS-CoV-2 Virus Like Particles (Ha-CoV2) Delta Plus (AY.2)

SARS-CoV-2, Virus Like Particles (Ha-CoV2) Delta Plus (AY.2) The hybrid alpha-pseudovirus for SARS-CoV-2 (Ha-CoV-2) is a newly developed SARS-CoV-2 virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid report expression (luciferase or GFP) in target cells (Hetrick et al., 2022). Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, Ha-CoV-2 is assembled with all 4 structural proteins (S, M, N, and E) of SARS-CoV-2, and contains a reporter genome derived from an alphavirusbased vector for rapid (6 hours) and robust expression of reporter genes. The alphavirus vector does not contain any of the structural proteins from the authentic virus and the alpha-pseudovirus particles are single-cycle. Spike Mutations: Spike T19R, Spike V70F, Spiked del157/158, Spike A222V, Spike K417N, Spike L452R, Spike T478K, Spike D614G, Spike P681R, Spike D950N Size:

25 X 200 µL ~100 wells/96 well plate
60x Conc. 5 x 100 µL

Reporter: Available with Firefly luciferase, GFP, or RFP Formulation & Storage: Store at -80°C. Intended for research use only, not for human, therapeutic, or diagnostic applications.

Price:

$1,490.00 – $2,350.00

SARS-CoV-2, Virus Like Particles (Alpha (B.1.1.7))

SARS-CoV-2 Virus Like Particles (Alpha (B.1.1.7)) The hybrid alpha-pseudovirus for SARS-CoV-2 (Ha-CoV-2) is a newly developed SARS-CoV-2 virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid report expression (luciferase or GFP) in target cells (Hetrick et al., 2022). Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, Ha-CoV-2 is assembled with all 4 structural proteins (S, M, N, and E) of SARS-CoV-2, and contains a reporter genome derived from an alphavirusbased vector for rapid (6 hours) and robust expression of reporter genes. The alphavirus vector does not contain any of the structural proteins from the authentic virus and the alpha-pseudovirus particles are single-cycle. Spike mutations: Spike A570D, Spike D614G, Spike D1118H, Spike H69del, Spike N501Y, Spike P681H, Spike S982A, Spike T716I, Spike V70del, Spike Y145del Size:

25 X 200 µL ~100 wells/96 well plate
60x Conc. 5 x 100 µL

Reporter: Available with Firefly luciferase, GFP, or RFP Formulation & Storage: Available upon request. Store at -80°C. Intended for research use only, not for human, therapeutic, or diagnostic applications.