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Flow Cytometry Services For Cellular Advanced Analysis

At IBT Bioservices, we offer exceptional flow cytometry services to support your research and diagnostic needs. Our state-of-the-art flow cytometry laboratory is equipped with a BD FACSymphony A3. We have developed several panels for mouse, human and nonhuman primates. 

Our Flow Cytometry Services provide a comprehensive range of analytical techniques and solutions to meet diverse requirements. Whether you are investigating cellular phenotypes, studying cell signaling pathways, or analyzing immune responses, our advanced flow cytometry technologies can provide valuable insights into your research.

High-Quality Analysis: Our cutting-edge flow cytometers offer multi-parameter analysis, allowing simultaneous measurement of multiple parameters, including cell surface markers, intracellular proteins, and DNA content. This enables comprehensive characterization and profiling of cell populations with high precision.

Customized Panel Design: We understand that each research project is unique, requiring specific markers and parameters. Our expert team collaborates closely with you to design customized antibody panels tailored to your experimental needs. We can also assist with flow panel design and optimization, customizing the assays to ensure optimal panel configurations and maximize the quality of data generated.

 

 

Why Choose Our Flow Cytometry Services?

IBT stands out for its expertise in delivering tailored flow cytometry solutions. Our services encompass everything from sample preparation to detailed data analysis, ensuring that each step is optimized for accuracy. By choosing IBT, clients gain access to experienced scientists, advanced flow cytometry instruments, and rigorous quality control standards, making us the ideal partner for reliable and insightful flow cytometry results.

 

Our Process

Our flow cytometry process is designed for efficiency and accuracy at every stage:

 

Consultation and Planning: We work with you to design custom panels and protocols that meet the unique requirements of your project.

 

Sample Preparation: Using precise and consistent protocols, we prepare samples to ensure data integrity and reliable phenotyping results.

 

Data Collection and Analysis: Leveraging high-throughput flow cytometers, we collect and analyze data with precision, providing comprehensive insights for each sample.

 

Quality Control: Our rigorous quality assurance measures guarantee the reliability and reproducibility of results across multiple assays.

 

This structured approach ensures that our flow cytometry services deliver consistent, high-quality data for your research.

 

Sample Preparation and Requirements

Proper sample preparation is essential for obtaining reliable flow cytometry data. We provide detailed instructions on sample collection, handling, and submission to maximize data quality. Our laboratory is equipped to handle a variety of sample types, including blood, tissue, and cell cultures, ensuring flexibility for various research applications. Each sample undergoes rigorous preparation protocols to maintain the highest standards in flow cytometry phenotyping and analysis.

Quality Assurance and Data Analysis

IBT BioServices places a strong emphasis on quality assurance throughout the flow cytometry process. From initial setup to final data analysis, our team follows strict quality control protocols to ensure accuracy and reproducibility. Our flow cytometry data analysis services include advanced gating strategies, quantitative analysis, and statistical evaluation, providing you with robust and interpretable results. We offer both raw data and fully analyzed reports, tailored to meet your specific needs.

 

Our Equipment and Technology

Our flow cytometry services are powered by advanced technology, featuring high-performance flow cytometers capable of multi-parameter analysis. This technology allows us to provide detailed cell population phenotyping, accurate quantification, and sophisticated data interpretation. With access to the latest flow cytometry instruments, we are equipped to handle both routine and complex analyses, ensuring high-quality results for each project.

Use Cases

Our flow cytometry services support a variety of applications, including:

Immunophenotyping: Comprehensive immune cell profiling to support immunology and oncology research.

Biomarker Discovery: Identification and quantification of biomarkers relevant to drug discovery and diagnostics.

Cell Cycle Analysis: Evaluation of cell cycle distribution and proliferation for cancer research.

 

Apoptosis Assays: Detection and analysis of apoptotic markers to study cell death mechanisms.

 

Cytokine Profiling: Multiplex analysis of cytokine expression for understanding immune responses.

 

These applications make our flow cytometry services ideal for researchers across multiple disciplines, providing accurate data and valuable insights.

 

Flow Cytometry Panel Design

Our team works closely with clients to design custom flow cytometry panels tailored to their specific research needs. With expertise in multi-parameter analysis, we help you select the optimal panel configurations and fluorochromes, ensuring precise and informative results. Our panel design services support efficient and targeted data collection, making it easier to interpret complex biological processes.

 


Ready to elevate your research with advanced flow cytometry services? Schedule a consultation with IBT BioServices to discuss your project requirements and learn how our custom solutions can support your flow cytometry needs.

 Full Panel

Antibody

Extracellular staining

CD62L – BUV395

CD4 – BUV805

CD8 – BV480

CD44 – BB515

TCR-gd-BUV661

CD3e-BV605

CD103-BV786

CD69-PE-Cy5

CD45 – AF700

L/D stain  Near IR
TNFa – AF647.   
Intracellular staining  Ki67- PerCP-Cy5.5
CD4 – BUV805
TNFa – PE-Cy7
IL-13 PE
IL-4 – BV650
IL-10 BV711
IL-22 APC
IFNg – BUV737
IL-17a – PE/Dazzle594
FoxP3 – eFluor 450
  • Data Analysis and Interpretation: We provide comprehensive data analysis and interpretation services to help you extract meaningful insights from your flow cytometry experiments. Our skilled bioinformaticians and scientists utilize advanced software tools to analyze complex datasets, identify subpopulations, and perform statistical analyses. We can also provide data analysis services for the acquired flow samples.
  • Quality Assurance: We follow stringent quality control measures throughout our workflow to ensure accurate and reproducible results. From sample preparation to data acquisition and analysis, we adhere to the highest standards to maintain data integrity and reliability.
  • Timely Delivery: We understand the importance of timely results in your research. Our streamlined processes and efficient workflow enable us to deliver your data within the agreed-upon timeframe, without compromising quality.
  • Collaboration and Consultation: Our team is dedicated to building strong collaborations with researchers and scientists. We offer consultation services to help you plan your flow cytometry experiments, optimize protocols, and troubleshoot any technical challenges you may encounter.       

Immune cell Phenotyping

  • T cell subsets, B cells, NK cells, Monocytes, Macrophages, Dendritic cells and Neutrophils

Functional assays

  • Intracellular cytokine staining

Species 

  • Mouse, NHP and Human

Tissues

  • Blood, Spleen, Lungs, Lymph nodes and Bone marrow

Whether you are a researcher in academia, a pharmaceutical company, or a clinical laboratory, our Flow Cytometry Services can accelerate your scientific endeavors and drive meaningful discoveries.

Contact us today to learn more about our comprehensive flow cytometry solutions and how we can support your research goals. Let us be your trusted partner in advancing the frontiers of scientific knowledge through the power of flow cytometry.

FAQ

What is flow cytometry phenotyping?

Flow cytometry phenotyping involves identifying and characterizing cell populations based on specific markers. This technique is widely used in immunology, oncology, and other research areas to analyze cell surface and intracellular molecules.

 

What is the typical turnaround time for flow cytometry analysis?

Turnaround time varies based on the complexity of the analysis and the number of samples. We aim to deliver results efficiently, keeping you informed at every stage.

 

Can I schedule a consultation to discuss my specific flow cytometry needs?
Yes, we offer consultations to discuss your project in detail and provide tailored solutions that align with your research goals.

 

Do you offer custom flow cytometry assays?
Absolutely. We provide fully customizable assays, including panel design, sample preparation, data acquisition, and analysis, to meet your unique research requirements.

 

Can I receive raw data as well as analyzed data?
Yes, we provide both raw data and fully analyzed reports, giving you flexibility in data interpretation and further analysis.

 

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