Custom Protein Expression Services
If you’re involved in biochemical research, drug discovery, structural biology, or other fields requiring specific proteins for your experiments, our custom protein production services offer a reliable solution tailored to your unique requirements. This page will provide you with valuable information about our services and how we can assist you in obtaining high-quality custom proteins for your scientific endeavors
Why Choose IBT for Protein Expression Services?
IBT BioServices is a trusted partner in protein expression and purification services. With our extensive experience in recombinant protein expression and advanced protein purification techniques, we deliver results that meet the most stringent industry standards. Our services are customized to support diverse research applications, from basic science to therapeutic development. By choosing IBT, you gain access to a team of skilled scientists and state-of-the-art facilities dedicated to excellence in protein expression and purification.
Our Process
Our protein expression services follow a structured, rigorous workflow to ensure high-quality results at every step:
Consultation and Construct Design: We begin with a comprehensive consultation to understand your specific needs. Our team then designs optimized constructs suitable for various expression systems, including codon optimization and inclusion of tags.
Protein Expression: We specialize in custom protein expression using various systems, such as E. coli, mammalian, and insect cells. Each system is chosen based on the specific requirements of your project to achieve optimal yield and functionality.
Protein Purification: We employ cutting-edge protein purification services, including affinity chromatography, ion exchange chromatography, and size exclusion chromatography. This ensures high purity and functionality for downstream applications.
Quality Control: Rigorous quality control measures, including SDS-PAGE, Western blotting, and mass spectrometry, are performed at every stage. This ensures that the final product meets the highest standards for purity, integrity, and identity.
Our structured process guarantees a smooth and efficient experience, making us an ideal choice for all your protein expression and purification needs.
Popular Custom Protein Expression Systems
IBT BioServices offers a range of protein expression systems, each selected based on the specific demands of your project:
E. coli Expression: Cost-effective and rapid production for straightforward proteins.
Mammalian Expression: Ideal for proteins requiring post-translational modifications, providing high functional activity.
Insect Cell Expression: Suitable for complex proteins that require glycosylation, offering high-quality yield.
Each system offers distinct advantages, and our team is ready to help you select the best option to meet your project goals.
Standard workflow of recombinant protein production
Example of a solubly expressed recombinant protein in E. coli. Recombinant protein expression can also be performed in mammalian and insect cells.
Cell Paste Resuspension
In the initial step, cell paste resuspension involves rehydrating the collected cell paste to prepare it for further processing. This step is crucial as it ensures that cells are in a manageable state for the following mechanical lysis.
Mechanical Lysis (Microfluidizer)
Mechanical lysis uses a microfluidizer to break open the cell walls, releasing intracellular proteins and other contents. This method provides a controlled environment, ensuring efficient cell disruption while preserving the protein’s structural integrity for subsequent purification.
Cation Exchange Chromatography (POROS50 HS)
Following lysis, the solution is passed through cation exchange chromatography using POROS50 HS resin, which binds proteins based on their charge. This step helps separate the desired proteins from other cellular materials, setting up a cleaner sample for the next purification phase.
Mixed Modal Chromatography Biorad Ceramic HTP
In this stage, mixed modal chromatography further purifies the sample by leveraging multiple binding interactions. Using Biorad Ceramic HTP, it selectively retains target proteins, enhancing purity and making the sample ready for final formulation.
Concentration/Diafiltration and Final Formulation
After chromatography, concentration and diafiltration processes adjust the protein concentration and remove unwanted salts. This formulation step is essential to stabilize the protein in a suitable buffer for downstream applications.
Characterization Assays
The final step, characterization assays, verifies the protein’s identity, purity, and functionality through analytical techniques. This ensures that the final product meets quality standards, completing the workflow from cell preparation to purified protein.
Standard workflow of recombinant protein production
IBT BioServices offers a range of protein expression systems, each selected based on the specific demands of your project:
E. coli Expression: Cost-effective and rapid production for straightforward proteins.
Mammalian Expression: Ideal for proteins requiring post-translational modifications, providing high functional activity.
Insect Cell Expression: Suitable for complex proteins that require glycosylation, offering high-quality yield.
Each system offers distinct advantages, and our team is ready to help you select the best option to meet your project goals.
Recombinant protein is purified using two column chromatography steps
Here are key features and components of our custom protein production:
Optimization of Expression Conditions
Scale-Up Capability
Documentation and Reporting
Confidentiality and IP Protection
Post-Production Support
OUR EXPERTISE
Comprehensive Protein Production Services
Customization and Consultation
Construct Design and Cloning
Expertise in construct design and cloning ensures the creation of optimized expression vectors suitable for the chosen host system, taking into account factors such as codon optimization and inclusion of tags.
Protein Purification Expertise
State-of-the-art purification techniques, such as affinity chromatography, size exclusion chromatography, and ion exchange chromatography, are employed to achieve high purity levels and preserve protein integrity.
Quality Control Measures
WHY CHOOSE IBT?
EXPERTISE
Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.
CUSTOMIZED SOLUTIONS
We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.
TIMELY RESULTS
We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.
QUALITY ASSURANCE
We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.
EXCEPTIONAL SUPPORT
Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.
FAq
What quality control measures are included?
We implement stringent quality control measures, including SDS-PAGE, Western blotting, and mass spectrometry, ensuring high purity and functional integrity of the expressed protein.
How much protein yield can I expect?
Yields vary based on the expression system and protein complexity. We work with you to set realistic yield expectations and optimize conditions to maximize protein output.
What is the typical timeline for protein expression projects?
Timelines depend on project complexity and system choice. However, we prioritize efficiency and aim to deliver results promptly while maintaining high quality.
Can you help with construct design?
Yes, our team offers comprehensive support in construct design, including codon optimization and tag inclusion, to ensure successful expression.
Do you offer scale-up for larger production needs?
Absolutely. We offer scale-up capabilities to accommodate larger production needs, providing flexibility for both small-scale studies and large-scale applications.
How should I ship my samples, and do you offer support for international clients?
We provide detailed instructions for sample preparation and shipping, ensuring safe transport. We also support international clients, offering guidance on regulatory and shipping requirements