We offer Quantitative PCR (qPCR) and Real-Time PCR (RT-PCR) tests as part of our extensive suite of antiviral supporting assays. Our molecular laboratories are equipped with the Bio-Rad CFX96 thermal cycler to run a variety of protocols. Our team of scientific experts at IBT have experience extracting DNA & RNA from a variety of samples types and collaborate closely with you to develop unique molecular solutions for your testing requirements.

RT-PCR & qPCR Services for Clinical & Preclinical Research

QUANTITATIVE ANALYSIS OF INFLUENZA A VIRUS RNA USING THE STANDARD CURVE METHOD AND QPCR AMPLIFICATION

Using the standard curve method, the absolute quantity of RNA in test samples is measured. The standard curve graph on the left was created by performing 5-fold serial dilutions of cDNA generated from RNA extracted from a stock of Influenza A (IFV-A)/Puerto Rico/8/1934. The x-axis represents the log values of DNA starting dilution, while the y-axis indicates the measured quantification cycle (Cq) value. The graph on the right shows the amplification of cDNA made from RNA extracted from samples.

QUANTITATIVE DETECTION AND SPECIFICITY ANALYSIS OF ZIKA VIRUS RNA USING QPCR AMPLIFICATION AND MELT CURVE ANALYSIS

The amplification plot below was generated from Zika virus-containing samples. Samples were serially diluted 5-fold and gene amplification of the Zika envelop protein was observed. On the right, the melt curve plot indicates the melting temperature of a target. This specific melt curve shows high specificity to the Zika envelope protein gene, indicated by the peaks isolated to 78°C.

OUR PATHOGENS

We have a large panel of strains available and the ability to develop the assay for previous and emerging influenza strains.

COMPREHENSIVE IN VITRO ASSAY SERVICES

CUSTOMIZED ASSAY DESIGN:

We understand that every research project is unique. Our experts work closely with you to design customized HAI assays tailored to your specific requirements, ensuring optimal sensitivity and specificity.

SAMPLE ANALYSIS:

Whether you need to analyze a large number of samples or perform a small-scale study, our laboratory is fully equipped to handle both high-throughput screening and low-volume testing. We offer quick turnaround times without compromising the quality and accuracy of the results.

QUALITY CONTROL:

We adhere to strict quality control protocols to maintain the integrity of our assays. Our team conducts regular internal quality assessments, and our processes are designed to meet or exceed industry standards, ensuring reliable and reproducible data.

DATA ANALYSIS AND REPORTING:

Our services extend beyond performing the assay. We provide comprehensive data analysis and interpretation to help you make informed decisions. Our reports are clear, concise, and include all the necessary details, allowing you to easily understand and present your findings.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE:

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL CUSTOMER SUPPORT:

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.