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Staphylococcus aureus Pneumonia Model

To establish pneumonia, mice are intranasally inoculated with a lethal or sub-lethal dose of Staphylococcus aureus USA300 strain.

Health checks are performed four times a day within the first 72 hours of infection and once per day until termination of the study. Weight is recorded once daily during the course of disease. Lower, non-lethal inoculum challenge can be performed for CFU determination over extended periods of time.
Meta-analysis of results for studies comparing experimental vaccine versus control groups in this model indicate improved survival among vaccinated mice.

KEY FEATURES

Animal Background: Challenge Route: Challenge Dose: Mean Time to Death:
BALB/C, CD-1 Intranasal Instillation (IN) Model Dependent Day 2
Standard Readouts: Other Readouts:
Weights, health and survival Bacterial Assays (MIC), ELISA, Luminex, Flow cytometry

Description: Female BALB/c mice were challenged with
Staphylococcus aureus USA300 NRS384 at both LD50 and
LD90 doses via intranasal (IN) instillation.

Description: Female BALB/c (n=10) mice were treated via the intraperitoneally (IP) with two antibodies 2- hours before intranasal (IN) instillation with 1x LD90 of Staphylococcus aureus USA300 NRS384. The animals treated with the control antibody succumbed to infection by day 2 post-infection.

S. aureus Strain

  • USA300
  • USA300

Isolate/ Subtype

  • LAC
  • NRS384