Microneutralization Assay Services to Assess Antiviral Activity

A Microneutralization (MN) Assay is used to measure the ability of serum or antibodies to neutralize the infectivity of a virus. This assay is a crucial tool in virology and immunology for assessing the presence and potency of neutralizing antibodies in biological samples.

Why Choose IBT for Microneutralization Assay Services?

At IBT Bioservices, we provide microneutralization assay services designed to deliver precise and reliable data for vaccine & therapeutic development, immunology research, and diagnostics. With a team of experts and access to a broad range of viral strains, we offer fully customized solutions tailored to your specific research goals. We maintain rigorous quality control and use state-of-the-art technology, ensuring the highest standards for every assay.

Our Process

At IBT Bioservices, we follow a well-defined process to ensure accuracy and efficiency in our microneutralization assays:

  1. Sample Preparation: We prepare the test articles for incubation with the virus.
  2. Virus-Test Articley Incubation: The virus is mixed with serially diluted test articles as per pre definiassay parameters  and incubated. The Sample/Virus mixture is then added to the cell mono-layer.
  3. Cytopathic Effect (CPE): The reduction of cytopathic effects is measured to determine the neutralizing potency of the test article.
  4. Staining and Analysis: Host cells are stained with crystal violet OR immunostained using a cell-based ELISA to determine inhibition of viral infectivity .
 

Influenza ELISA

In addition to microneutralization assays, we offer Influenza ELISA services. ELISA (Enzyme-Linked Immunosorbent Assay) is used to measure the concentration of specific antibodies against influenza viruses, providing important data on immune response and vaccine efficacy. Our Influenza ELISA services are an integral part of our virology research offerings, helping clients gain insights into influenza immunity and antibody titers.

Our Pathogens

We have a large panel of strains available for testing, including:

    • Influenza A (H1N1 & H3N2)
    • Influenza B (Yamagata & Victoria)
    • Dengue Virus (Serotypes 1-4)
    • Zika Virus
  • Chikungunya Virus
  • Herpes Simplex Virus (HSV-1 & HSV-2)
  • Respiratory Syncytial Virus (RSV-A & RSV-B)

We also offer custom assay development for emerging pathogens, ensuring you stay at the forefront of vaccine and antiviral research.

Antibodies tested in a microneutralization assay against Influenza B/Brisbane.60/2008

Plates were seeded with MDCK cells one day prior to the assay. Antibody 1, Antibody 2, and the Internal Control were serially diluted to Concentrations 1-8 and incubated with Influenza B/Brisbane/60/2008 at a predetermined MOI. The antibody/virus mixture was added to the cells and incubated at 35°C for several days until sufficient cytopathic effect (CPE) was observed in the Virus Only control wells. Cells were stained with crystal violet. Viable cells stain bright purple.

Antibodies tested in a microneutralization assay against Influenza B/Brisbane.60/2008

Neutralization is observed for the first few concentrations for both antibodies. Antibody A reaches a 50% neutralizing titer (NT50) at a lower concentration and demonstrates more neutralizing potency against IFV-B/Brisbane/60/2008 than Antibody B. The recorded NT50 of the internal control fell within the expected range.

The Microneutralization Assay is based on the principle that neutralizing antibodies have the capacity to inhibit or block the ability of a virus to infect host cells. By measuring the reduction in viral infectivity in the presence of specific antibodies, researchers can determine the neutralizing capability of these antibodies.

OUR PATHOGENS

We have a large panel of strains available and the ability to develop the assay for previous and emerging influenza strains.

We offer an extensive range of viruses for your testing needs and can develop custom microneutralization assays to meet your specific research objectives.

Respiratory Viruses – Influenza, RSV, ADV

Arboviruses – Dengue 1-4, CHKV, JEV, LACV, RVF, YFV, ZIKV

Herpes Viruses – CMV, HSV-1, HSV-2, CMV

Enteric Viruses – CVA, Enteroviruses, Echoviruses

Pseudoviruses – Filoviruses, SARS-CoV-2

These assays are invaluable tools for understanding the immune response to viral infections and the assessment of vaccines. They provide important information about the presence and potency of neutralizing antibodies, which are crucial for protection against many viral diseases.

Microneutralization Assays

Applications of Microneutralization Assays

Immunology Research

Microneutralization Assays are used to study the immune response to viral infections, vaccine efficacy, and the development of immunity in individuals or populations.

Vaccine Development

 These assays play a critical role in evaluating the effectiveness of vaccines by assessing their ability to induce neutralizing antibodies.

Epidemiology

MN assays are employed in seroepidemiological studies to estimate the prevalence of specific antibodies within a population, providing insights into the history and prevalence of viral infections.

Diagnostic Testing

Some MN assays are used for diagnostic purposes, especially for confirming the presence of antibodies to specific viruses, such as in the case of diagnosing certain viral diseases like measles or mumps.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL SUPPORT

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.

FAQ

What is Microneutralization?
Microneutralization is an assay used to determine the ability of test articles to neutralize viruses and prevent them from infecting host cells. It measures the potency of neutralizing antibodies by reducing the virus’s ability to cause infection.

What is the difference between HAI and Microneutralization assay?
Both assays measure the presence of neutralizing antibodies, but HAI (Hemagglutination Inhibition Assay) detects antibodies that prevent the agglutination of red blood cells, while the Microneutralization Assay measures antibodies that inhibit viral infection of host cells. MN assays are typically more sensitive and can be used for a broader range of viruses.

What is a NAb assay?
A NAb (Neutralizing Antibody) assay measures the ability of antibodies to neutralize a virus by inhibiting its infectivity in vitro. The assay provides crucial information about the potency of antibodies, which is especially important in evaluating vaccine responses and immune protection.

What cell type do we use in a Microneutralization assay?
We most commonly use MDCK, Vero & A549 cells  for microneutralization assays, but other cell types may be used depending on the pathogen being studied. The selection of cell type depends on the host specificity of the virus. 

What is the difference between PRNT and MNT?
PRNT (Plaque Reduction Neutralization Test) and MNT (Microneutralization Test) are both neutralization assays, but they differ in methodology. PRNT measures the reduction of viral plaques (clear zones of cell lysis), while MNT measures the reduction of cytopathic effects (CPE) in host cells. MNT is generally faster and more scalable for high-throughput testing compared to PRNT.

Contact us today at services@ibtbioservices.com to learn more about our Microneutralization Assay Services and how we can support your research in virology, immunology,vaccine or therapeutic development.



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