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Staphylococcus aureus Bacteremia Model

Bacteremia in mice can be established by challenging mice via the intraperitoneal (IP) or intravenous (IV) route with US300 strain of interest followed by monitoring of health, weight and survival.

Meta-analysis of results for studies comparing experimental vaccine versus control groups in this model indicate improved survival among vaccinated mice.

KEY FEATURES

Animal Background: Challenge Route: Challenge Dose: Mean Time to Death:
BALB/C, CD-1 Intraperitoneal (IP), Intravenous (IV) Strain Dependent Strain and Route Dependent
Standard Readouts: Other Readouts:
Weights, health and survival Bacterial Assays (MIC), ELISA, Luminex, Flow cytometry, Bacterial load in organs

Description: Female BALB/c (n=75) mice were challenged with varying doses of USA300 NRS384 via the intravenous (IV) route.

Description: Female BALB/c (n=190) mice were challenged with varying doses of USA300 NRS384 via the intraperitoneal (IP) route.

Description: Female BALB/c (n=30) mice were challenged with 1x LD90 of USA300 NRS384 via the intraperitoneal (IP) route. All animals were challenged on day 0 and received treatment via the IP route twice daily for five days.

S. aureus Strain

  • USA300
  • USA300

Isolate/ Subtype

  • SF8300
  • NRS384