Proteins

Custom Protein Production Services

If you’re involved in biochemical research, drug discovery, structural biology, or other fields requiring specific proteins for your experiments, our custom protein production services offer a reliable solution tailored to your unique requirements. This page will provide you with valuable information about our services and how we can assist you in obtaining high-quality custom proteins for your scientific endeavors

Standard workflow of recombinant protein production

Example of a solubly expressed recombinant protein in E. coli. Recombinant protein expression can also be performed in mammalian and insect cells.

Recombinant protein is purified using two column chromatography steps

First step, cell lysates is loaded onto the cation exchange column and eluted by step elution with high salt. The desired protein is eluted in fraction 2, diluted two-fold and loaded on to a mixed mode column. The protein is eluted by increasing phosphate concentration. The desired protein is eluted in fraction 2.

This assay tests for hemagglutinin (HA)-specific antibodies against influenza virus (INFV). Serial dilutions of the TA starting are incubated with a standardized amount of IFV antigen and Turkey Red Blood Cells (TRBCs) in 96-well plates. In the absence of antibodies against influenza hemagglutinin (HA) glycoprotein, the HA protein agglutinates TRBCs. Hemagglutination is inhibited in the presence of HA-specific antibodies, which will bind to the antigenic sites on the HA protein. The number of antibodies present in a sample is quantified as an HA titer, given as the highest dilution of serum that completely inhibits hemagglutination. IBT’s method is standardized to the WHO testing protocol and can use live virus for testing.

Here are key features and components of our custom protein production:

Optimization of Expression Conditions

Scale-Up Capability

Documentation and Reporting

Confidentiality and IP Protection

Post-Production Support

With years of experience in the field, our team of skilled scientists and technicians are well-versed in conducting HAI assays for a wide range of pathogens, including influenza viruses, respiratory syncytial virus (RSV), Herpes Simplex Virus (HSV-1 & HSV-2), etc. We utilize state-of-the-art equipment and follow stringent quality control measures to ensure precise and reproducible results.

OUR EXPERTISE

Comprehensive Protein Production Services

Customization and Consultation

Services begin with in-depth consultations to understand the client’s specific needs, including the desired protein, its intended application, any modifications required, and the preferred expression system.

Construct Design and Cloning

Expertise in construct design and cloning ensures the creation of optimized expression vectors suitable for the chosen host system, taking into account factors such as codon optimization and inclusion of tags

Protein Purification Expertise

State-of-the-art purification techniques, such as affinity chromatography, size exclusion chromatography, and ion exchange chromatography, are employed to achieve high purity levels and preserve protein integrity.

Quality Control Measures

Stringent quality control measures are implemented throughout the production process, involving analytical techniques like SDS-PAGE, Western blotting, mass spectrometry, and other methods to verify protein identity, purity, and integrity.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE:

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

CUSTOMER SUPPORT:

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.

IN-VITRO Services

ANIMAL SERVICES

By Diseases Target