Zika Virus Efficacy Models

Zika Virus (ZIKV) is a mosquito-borne flavivirus associated with the recent outbreak of febrile disease across the Americas.

Zika infection causes Zika fever and infection during pregnancy is hypothesized to cause microcephaly in neonates. The IFN / and IFN receptor deficient AG129 mouse model using ZIKV FSS13025 strain is based on previously described model (Rossi, et al., 2016). AG129 mice aged 6-8 week challenged with FSS13025 strain have a mean time to death of 11-12 days post infection. This model provides a biologically relevant approach for studying ZIKV infection without relying on intracerebral inoculation. It has several advantages, including the ability to induce lethal and non-lethal infections without requiring mouse-adapted ZIKV strains.
Additionally, consistent symptoms are associated with disease development, making it a well-suited model for screening anti-viral compounds and evaluating vaccine candidates. IBT provides models using the FSS13025 and PRVABC59 Asian Pacific lineage strains.


Animal Background: Challenge Route: Mean Time to Death: Standard Readouts:
AG129 Subcutaneous (SC) Strain dependent Weights, health and survival
Other Readouts
CPE Assay, HAI Assay, PRNT, Microneutralization assay, Pseudovirus neutralization assay, ELISA, Luminex, Flow cytometry, viremia assessment of various organs including the reproductive system