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Staphylococcus aureus Skin and Soft Tissue Infection

MRSA Skin Infection Model. S. aureus suspension is administered intradermally (ID) or subcutaneously (SC) and BALB/c are monitored daily for weight loss.

Lesions are measured daily for 14 days post- infection using calipers. Vaccinated or mice treated with therapeutics have shown protection from lesion formation (PMID: 33777005). Insights gained from rodent studies can be translated into clinical practice to improve the diagnosis, treatment, and prevention of Staph aureus SSTIs in humans.
Mouse models provide a controlled environment for testing hypotheses and evaluating potential interventions before moving to human studies.

KEY FEATURES

Animal Background: Challenge Route: Peak Lesion Size: Standard Readouts:
BALB/C, CD-1 Intradermal (ID) Strain dependent Weights, health, survival, lesions, photographs
Other Readouts:
Bacterial Assays (MIC), ELISA, Luminex, Flow cytometry

Description: Two groups of Balb/C mice were infected with 1.00E+07 CFU/mouse of Staphylococcus aureus USA300 (NRS384) on day 0 via the intradermal (ID) route. One group received the experimental vaccine while the other group served as infection only control. On Day 49 of the study, both groups were infected with 1.00E+08 CFU/mouse and monitored for an additional 14 days. Lesion areas of vaccinated and non-vaccinated mice after primary and secondary infection.

Description: Two groups of Balb/C mice were infected with 1.00E+07 CFU/mouse of Staphylococcus aureus USA300 (NRS384) on day 0 via the intradermal (ID) route. One group received the experimental vaccine while the other group served as infection only control. On Day 49 of the study, both groups were infected with 1.00E+08 CFU/mouse and monitored for an additional 14 days. Lesion areas of vaccinated and non-vaccinated mice after primary and secondary infection.

Description: Two groups of Balb/C mice were infected with 1.00E+07 CFU/mouse of Staphylococcus aureus USA300 (LAC) on day 0.
One group received a novel vaccine candidate while the other group served as control receiving BCA.

S. aureus Strain

  • USA300
  • USA300
  • USA100
  • USA1000

Isolate/ Subtype

  • LAC
  • NRS384
  • NRS699
  • PVL