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Influenza Efficacy Models

Influenza viruses (INFV) are segmented RNA viruses that cause seasonal influenza epidemics and pandemics.

Influenza A can infect and be transmitted between birds and some mammals (e.g. pigs and humans). Influenza B only infects humans and seals. Influenza infection poses a severe threat to public health and the agricultural economy. This virus mutates quickly by both antigenic drift and reassortment requiring the development and reformulation of new vaccines each year. Influenza strains resistant to almost all known drugs have been identified, making drug development a critical pursuit.
Concerns about INFV infection are further heightened by the potential for transmission of highly pathogenic avian strains (HPAI) to humans. IBT Bioservices has developed several mouse models of Influenza infection wherein mice are inoculated intranasally with an LD90 dose of virus.

KEY FEATURES

Animal Background Challenge Route Mean Time to Death
Balb/C, C57Bl6 Intranasal (IN) 7 Days (Strain Dependent)
Peak Viremia Standard Readouts Other Readouts
4-5 Days Weights, Health And Survival CPE Assay | HAI Assay | PRNT | Microneutralization assay | Pseudovirus neutralization assay | ELISA | Luminex | Flow cytometry | lung and BAL viremia | Temperature (internal microchips)

Description: Female BALB/c mice were treated with two antibodies 1-hour prior to inoculation with LD90 of Influenza H3N2 A/Hong Kong/1/68 via the intranasal (IN) route. The mean time of death for this virus strain falls on day 6.

Description: Female BALB/c mice were treated with two antibodies 24-hours before inoculation with 1.00E+05 PFU/animal of Influenza B/Florida/04/2006 (Yamagata
lineage) via the intranasal (IN) route. The mean time of death for this virus strain falls on day 7.

Subtype/Lineage

  • A(H1N1)
  • A(H1N1)
  • A(H1N1)
  • A(H1N1)
  • A(H3N2)
  • A(H3N2)
  • Yamagata Lineage
  • Victoria Lineage

Virus Strain

  • A/Texas/36/91
  • A/PUERTO RICO/8/34
  • A/Perth/261/2009 (H25Y) Tamiflu Resistant
  • A/California/07/09
  • A/Hong Kong/01/68
  • A/VICTORIA/03/75
  • B/Florida/04/06
  • B/Brisbane/60/09