If you’re involved in biochemical research, drug discovery, structural biology, or other fields requiring recombinant antibody production for your experiments, our custom antibody production services offer a reliable solution tailored to your unique requirements. Recombinant antibody expression can be performed by transient transfection in HEK293 cells or stable transfection in CHO cells. Cells can be grown in shake flask, spinner flask and bioreactors (up to 10L) and purified by Protein A chromatography. This page will provide you with valuable information about our services and how we can assist you in obtaining high-quality custom proteins for your scientific endeavors.
Cell media is collected, and the recombinant antibody, purified by protein A, is immobilized on a solid support. CHO cell media was loaded onto a protein A column, washed, and then eluted by low pH. Blue line is the A280. Antibody is eluted using a low pH buffer. The eluted antibody is immediately neutralized by high pH buffer. The antibody is then dialyzed into final storage buffer.
For SDS-PAGE, the purified antibody was electrophoresed on a reducing SDS-PAGE to separate heavy and light chains. MW=Protein Molecular Weight Markers. Lane 1 is Antibody at 1µg/lane. For SEC-HPLC, the purified antibody (50 µg) was loaded onto SEC-column. Elution profile shows 100% purity.
Custom antibody production services offer tailored solutions for the synthesis and purification of antibodies according to the unique needs of researchers, pharmaceutical companies, and other biotechnology entities. These services provide a streamlined and efficient way to obtain antibodies for various applications, such as structural biology, drug discovery, functional studies, and diagnostic development.
With years of experience in the field, our team of skilled scientists and technicians are well-versed in conducting antibody production. We utilize state-of-the-art equipment and follow stringent quality control measures to ensure precise and reproducible results.
Services begin with in-depth consultations to understand the client’s specific needs, including the desired protein, its intended application, any modifications required, and the preferred expression system.
Expertise in construct design and cloning ensures the creation of optimized expression vectors suitable for the chosen host system, considering factors such as codon optimization and inclusion of tags.
State-of-the-art purification techniques, such as affinity chromatography, size exclusion chromatography, and ion exchange chromatography, are employed to achieve high purity levels and preserve protein integrity.
Stringent quality control measures are implemented throughout the production process, involving analytical techniques like SDS-PAGE, Western blotting, mass spectrometry, and other methods to verify protein identity, purity, and integrity.
Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.
We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.
We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly
We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.
Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.
