Ebola Virus like particles (HA-EBOV) Sudan (Genebank: YP_138523.1)
A novel rapid hybrid alpha-pseudovirus for Ebolaviruses (EBOV) (HA-EBOV) that are available for your initial testing from the Sudan Ebola strain (Genebank: YP_138523.1). These pseudoviruses are BSL2 safe and ready to use for studying viral entry. HAEBOV particles are pseudoviruses assembled from the structural proteins of the filovirus glycoprotein (GP), VP40 matrix protein, and the Nucleoprotein (NP) and package an alphaviral vector for reporter gene expression. The alpha-pseudoviruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs.
Size:
1X concentrated 25 x 200 µL ~100 wells/96 well plate
Reporter: Available with Firefly luciferase, GFP, or RFP
Formulation & Storage: Available upon request. Store at -80°C.
Applications
Rapid NiV pseudovirus transduction of target cells for viral entry and functional studies
Anti-NiV drug screening
Anti-NiV neutralizing antibody screening
Intended for research use only, not for human, therapeutic, or diagnostic applications.
Ebola Virus like particles (HA-EBOV) Zaire (Genebank: KJ660346.2)
A novel rapid hybrid alpha-pseudovirus for Ebolaviruses (EBOV) (HA-EBOV) from the Zaire strain that are available for your initial testing. These pseudoviruses are BSL2 safe and ready to use for studying viral entry. HAEBOV particles are pseudoviruses assembled from the structural proteins of the filovirus glycoprotein (GP), VP40 matrix protein, and the Nucleoprotein (NP) and package an alphaviral vector for reporter gene expression. The alpha-pseudoviruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs.
Size:
1X concentrated 25 x 200 µL ~100 wells/96 well plate
Reporter: Available with Firefly luciferase, GFP, or RFP
Formulation & Storage: Available upon request. Store at -80°C.
Applications
Rapid NiV pseudovirus transduction of target cells for viral entry and functional studies
Anti-NiV drug screening
Anti-NiV neutralizing antibody screening
Intended for research use only, not for human, therapeutic, or diagnostic applications.
ELISA kit for the quantitative measurement of Ebola Virus soluble glycoprotein (sGP) in mouse and non-human primate sera. ELISA kit contains all components necessary for running of the assay.
Components:
Capture Antibody: Anti-EBOV sGP
Standard: EBOV sGP
Secondary Antibody: Anti-EBOV sGP
Detection Reagent: Streptavidin
Substrate: TMB
Storage: Keep container tightly closed in a dry and well-ventilated place. Hygroscopic.
Virus-like Particles (VLP’s) expressing recombinant Ebola virus (EBOV) glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40).
These VLP’s mimic Ebola virus but do not contain genetic material, thus are not infectious and make an ideal candidate as a vaccine and tool to enhance filovirus research.
Formulation & Storage: Supplied in PBS (supplemented with arginine and glutamic acid). Store at 2-3 weeks at -20°C, -80°C long term. Subaliquoting is recommended to avoid repeated freeze/thaw cycles.
Pathogen Strain: Zaire
Purification: Column chromatography
Expression: SF9 cells
Please note: After thawing, VLP’s tend to sediment to bottom of the tube and appear as aggregate pellet. Resuspend by gentle vortexing or pipetting up and down
Certificate of analysis: A hardcopy of datasheet is sent along with the products. Please refer to it for detailed information.
