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rVSV pseudotyped Marburg Virus GP (Angola Strain)

Recombinant Vesicular Stomatitis Virus pseudotyped MARV-Angola glycoprotein (rVSV pseudotyped MARVAngola GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Marburg virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment. Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C. Notes & Usage Guidelines:
  • Recommended Dilution: 1: 800
  • Luciferase Units for Assays: 30,000
  • Reporter: Luciferase
  • Cat No:
  • 1004-001
  • Product Type:
  • Pseudovirus

Price:

$656.25

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rVSV pseudotyped Sudan Virus GP (Boniface Strain)

Recombinant Vesicular Stomatitis Virus pseudotyped Sudan glycoprotein (rVSV pseudotyped SUDV GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV pseudotypes containing the envelope glycoprotein of Sudan virus. Since the infectivity of rVSV pseudotyped SUDV GP is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment. Infectivity and neutralization of infectivity can be measured by luciferase activity.
  • Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C.
  • Notes & Usage Guidelines:
  • Recommended Dilution Range: 1: 40-1:80
  • Luciferase Units for Assays: 20,000-40,000
  • Cat No:
  • 1002-001
  • Product Type:
  • Pseudovirus

Price:

$656.25

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rVSV pseudotyped SUDV GP (Gulu Strain)

Recombinant Vesicular Stomatitis Virus pseudotyped Sudan glycoprotein – Gulu Strain
  • Cat No:
  • 1002-002
  • Product Type:
  • Pseudovirus

Price:

$656.25

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rVSV-pseudotyped Ebola virus GP (rVSV-EBOV-GP)

Recombinant Vesicular Stomatitis Virus (rVSV) pseudotyped with Ebola virus glycoprotein (rVSV-EBOV-GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV-pseudotyped virus containing the envelope glycoprotein of Ebola virus. Since the infectivity of pseudotyped virus is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment.
  • Formulation & Storage: Supplied in EMEM™ (Gibco) supplemented with 1% Fetal Bovine Serum, Lglutamine and Penicillin/Streptomycin. Store at -80°C.
  • Notes & Usage Guidelines:
  • Recommended Dilution: 1:40
  • Luciferase Units for Assays: 30,000
  • Reporter: Luciferase
  • Cat No:
  • 1001-001
  • Product Type:
  • Pseudovirus

Price:

$656.25

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Pan-filovirus Chimeric anti-GP mAb

Pan-filovirus Chimeric anti-GP mAb

Mature, recombinant, His-tagged Ebola virus Glycoprotein minus the transmembrane domain (EBOV rGPΔTM) is supplied as a purified protein. Ebola Virus Strain: Zaire Produced in stably transfected Drosophila Schneider 2 (S2) cells Contains His-Tag Purified by FPLC Ebola virus GPΔTM (S2) is intended for research use only

Recombinant Zaire Ebola virus GPΔTM (S2) – 500ug

Mature, recombinant, Ebolavirus Glycoprotein minus the transmembrane domain (Ebola virus GPΔTM (sf9)) Strain: Zaire produced in Sf9 insect cells using baculovirus for expression Contains His-Tag Supplied as purified protein Purified by FPLC.

Recombinant Zaire Ebola virus GPΔTM (Sf9) – 500ug

Viral Titration: Serially diluted pseudoviruses in EMEM™ in a 2-fold series were added to Vero cells seeded in a 96-well plate at a seeding density of 6E4 cells/well. Cells with media alone were used as control. After 24 h, the infection was monitored using the Luciferase Assay System (Promega, Madison, WI, USA). Viral titer was calculated using a dilution that resulted in a Relative Luciferase Unit (RLU). The RLU value recommended for this product is based readout from the BioTek Cytation™ instrument. Absolute RLU obtained by the user may vary based on the user’s instrument & handling or luciferase assay system.

rVSV-pseudotyped Ebola virus GP (rVSV-EBOV-GP)

Recombinant EBOV rGPΔTM: SDS-PAGE and Western Blot Data: (A) SDS-PAGE and stain demonstrating 5 g, 1 g (Lanes 1 and 2 respectively) of EBOV rGPΔTM HA-Tag protein under denaturing and reducing conditions. MW denotes Novex Sharp prestained protein markers. (B) Western blot detection of EBOV rGPΔTM at 500 ng, 100 ng, and 50 ng (Lanes 1-3). EBOV rGPΔTM was detected using IBT’s monoclonal antibody 4F3 (Catalog # 0201-020) at 0.5 g/mL and anti-mouse IgG-HRP conjugate, followed by TMB substrate.

Recombinant Ebola Virus rGPΔTM (Mammalian)

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