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Human drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these HEK293 cells and used for high-throughput applications. The transporters translocate substrates into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescent or LC-MS detection. Immunogen / Target: Human Multidrug Resistance Protein 8 (MRP8) Notes & Usage Guidelines: Protein Concentration: 1.5 mg/mL Volume: 4×500μL Expiration date: 2 years from the date of shipping Unit Size: 100 reactions / kit (96-wells format) Refreeze: Snap freeze vesicles with liquid nitrogen; store at-80°C

Human drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these HEK293 cells and used for high-throughput applications. The transporters translocate substrates into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescent or LC-MS detection. Immunogen / Target: Human Multidrug Resistance Protein 3 (MRP3) Notes & Usage Guidelines: Protein Concentration: 1.5 mg/mL Volume: 4×500μL Expiration date: 2 years from the date of shipping Unit Size: 100 reactions / kit (96-wells format) Refreeze: Snap freeze vesicles with liquid nitrogen; store at-80°C

Human drug efflux transporter vesicles are inside-out HEK293 membrane vesicles prepared for high-throughput, ATP-dependent substrate transport assays with detection by radiolabel, fluorescence, or LC-MS. Immunogen / Target: Blue Salt Export Pump (BSEP) Notes & Usage Guidelines: Protein Concentration: 1.5 mg/mL Volume: 4 x 500uL Unit Size: 100 reactions / kit (96-wells format) Expiration Date: 2 years from date of shipping Refreeze: Snap freeze vesicles with liquid nitrogen; store at -80°C