Dengue Virus Efficacy Models

This unique lethal model uses a non-mouse-adapted strain of DENV serotype 2 (D2Y98P), isolated from a DENV-infected patient in Singapore, which is highly infectious in AG129 mice.

The model provides consistent clinical manifestations of disease similar to those observed in human cases (Tan, et al., 2018). The DENV model has several advantages. Firstly, a low-challenge dose of the virus leads to transient asymptomatic infection followed by death a few days after viral clearance.
Secondly, it shows increased vascular permeability without morphological damage of the capillary endothelium, which is comparable to human disease. Finally, three vital pro-inflammatory cytokines implicated in Dengue Hemorrhagic Fever (DHF) are significantly elevated in D2Y98P-infected AG129 mice.


Animal Background: Challenge Route: Mean Time to Death: Standard Readouts:
AG129 Strain dependent Strain dependent Weights, health and survival
Other Readouts
CPE Assay, HAI Assay, PRNT, Microneutralization assay, Pseudovirus neutralization assay, ELISA, Luminex, Flow cytometry, lung and BAL viremia, Temperature (internal microchips)


  • DENV-2 D2Y98P
  • DENV-2 S221

Inoculation Route

  • Subcutaneous (SC)
  • Intravenous (IV)