An animal model is a non-human species used in biomedical research because it can mimic aspects of a biological process or disease found in humans.
Disease-to-target discovery is the process of identification and early validation of targets involved in a disease.
Animal Background: | Age Ranges: | Inoculations: | Therapeutic Agents: |
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Mice, Rats, Cotton rats, Guinea Pigs, Hamsters | Neonates to elderly | BSL-2 pathogens, peptides, small molecules, etc. | antibodies, small molecules, adjuvant mediated, new constructs, etc. |
Housing: | Treatment Administration Routes: | Readouts: | Regulation: |
Specialized housing for Immunocompromised mice, double filter Individual ventilated cages and racks | Intravenous, Intramuscular, Subcutaneous, Footpad, Intradermal, Oral gavage, Retro-orbital, Nebulizer, Sublingual | Animal health, weight, and survival data, CPE Assay, HAI Assay, PRNT, Microneutralization assay, Pseudovirus neutralization assay, ELISA, Luminex, Flow cytometry, lung and BAL viremia, Temperature (internal microchips) | Attending veterinarian, engaged IACUC committee |
Description: INFV H1N1 A/Puerto Rico/08/34 dose determination study in BALB/C mice when treated with 20mg/kg of oseltamivir phosphate twice daily for five days. Meta-analysis of seven independent studies (n=45).
Description: 10 mice were inoculated via intranasal instillation with 1.00E+05 PFU/mouse of A/Perth/261/2009 MH25Y (oseltamivir phosphate resistant strain). On day 4 post-infection, all animals were sacrificed, and lungs were harvested. Lungs were homogenated and final supernatants were titrated via plaque assay. The passage 1 (P1) lung supernatants were then used to infect new BALB/C mice.