Western Blot

Western Blot Services

At IBT Bioservices, we offer Western blot services to support your protein analysis needs. Western blotting, also known as immunoblotting, is a widely used technique for the detection and quantification of proteins in complex biological samples. Our Western blot services provide accurate and reliable results for protein expression, post-translational modifications, protein-protein interactions, and more.

Western Blot – Screening Antibodies for their ability to detect linear epitopes

Screening Human antibodies against Staphylococcus Aureus Toxin LukAB for reactivity by western blot. LukAB toxin was denatured prior to SDS-PAGE analysis. An anti-human Fc detecting secondary antibody conjugated to horseradish peroxidase (HRP) was used for detection of primary antibodies. These reagents were further used as reagents for identity assays required for vaccine manufacturing.

Customized Assay Design: We understand that each research project has unique requirements, and we tailor our Western blot services to meet your specific needs. Our team of experienced scientists works closely with you to design and optimize the assay conditions, including the choice of antibodies, sample preparation methods, electrophoresis conditions, and detection systems.

We Have A Large Panel Of Strains Available And The Ability To Develop The Assay For Previous And Emerging Influenza Strains.

Influenza A (H1N1)
/Puerto Rico/8/34

Influenza A (H3)
A/Delaware/01/2021

Influenza A (H1N1) Pdm09
A/Victoria/2570/2019

Influenza B, Yamagata Lineage
(B/Phuket/3073/2013)

Influenza B, Victoria Lineage (B
/Michigan/01/2021)

At IBT Bioservices, we are dedicated to providing you with reliable and innovative Western blot services. Our state-of-the-art facilities, cutting-edge technologies, and experienced scientific team make us the preferred partner for academic researchers, pharmaceutical companies, and biotechnology firms.

OUR EXPERTISE

Customized Assay Design:

We understand that each research project has unique requirements, and we tailor our Western blot services to meet your specific needs. Our team of experienced scientists works closely with you to design and optimize the assay conditions, including the choice of antibodies, sample preparation methods, electrophoresis conditions, and detection systems.

Sample Preparation and Protein Extraction:

We provide assistance with sample preparation and protein extraction from a variety of sample types, including cell lysates, tissue homogenates, blood, and other biological fluids. Our expertise ensures the isolation of high-quality proteins for accurate Western blot analysis.

Gel Electrophoresis:

We perform sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to separate proteins based on their molecular weight.

Protein Transfer:

After gel electrophoresis, we perform protein transfer onto a membrane using semi-dry or wet transfer methods. This step ensures the efficient transfer of proteins from the gel onto a membrane for subsequent antibody detection.

Antibody Selection and Detection

We offer a wide range of high-quality primary antibodies for target protein detection. Our experts assist in antibody selection based on your research needs. We utilize various detection methods, including chemiluminescence, fluorescence, or chromogenic detection systems, to visualize and quantify the protein bands of interest.

Data Analysis and Interpretation:

Our team of experts provides comprehensive data analysis and interpretation for Western blot results. We utilize advanced software and image analysis tools to quantify protein expression levels, assess band intensities, and perform statistical analysis. We deliver clear and concise reports that highlight key findings, helping you understand and communicate your research outcomes effectively.

Quality Control and Compliance:

At IBT we adhere to strict quality control measures. We implement standardized protocols, and ensure accurate and reproducible results. Your research is in safe hands with our commitment to quality and compliance.

COMPREHENSIVE IN VITRO ASSAY SERVICES

CUSTOMIZED ASSAY DESIGN:

We understand that every research project is unique. Our experts work closely with you to design customized HAI assays tailored to your specific requirements, ensuring optimal sensitivity and specificity.

SAMPLE ANALYSIS:

Whether you need to analyze a large number of samples or perform a small-scale study, our laboratory is fully equipped to handle both high-throughput screening and low-volume testing. We offer quick turnaround times without compromising the quality and accuracy of the results.

QUALITY CONTROL:

We adhere to strict quality control protocols to maintain the integrity of our assays. Our team conducts regular internal quality assessments, and our processes are designed to meet or exceed industry standards, ensuring reliable and reproducible data.

DATA ANALYSIS AND REPORTING:

Our services extend beyond performing the assay. We provide comprehensive data analysis and interpretation to help you make informed decisions. Our reports are clear, concise, and include all the necessary details, allowing you to easily understand and present your findings.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE:

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL SUPPORT:

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.

IN-VITRO Services

ANIMAL SERVICES

By Diseases Target