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Microneutralization Assay Services

A Microneutralization (MN) Assay is used to measure the ability of serum or antibodies to neutralize the infectivity of a virus. This assay is a crucial tool in virology and immunology for assessing the presence and potency of neutralizing antibodies in biological samples.

Antibodies tested in a microneutralization assay against Influenza B/Brisbane.60/2008

Plates were seeded with MDCK cells one day prior to the assay. Antibody 1, Antibody 2, and the Internal Control were serially diluted to Concentrations 1-8 and incubated with Influenza B/Brisbane/60/2008 at a predetermined MOI. The antibody/virus mixture was added to the cells and incubated at 35°C for several days until sufficient cytopathic effect (CPE) was observed in the Virus Only control wells. Cells were stained with crystal violet. Viable cells stain bright purple.

Antibodies tested in a microneutralization assay against Influenza B/Brisbane.60/2008

Neutralization is observed for the first few concentrations for both antibodies. Antibody A reaches a 50% neutralizing titer (NT50) at a lower concentration and demonstrates more neutralizing potency against IFV-B/Brisbane/60/2008 than Antibody B. The recorded NT50 of the internal control fell within the expected range.

The Microneutralization Assay is based on the principle that neutralizing antibodies have the capacity to inhibit or block the ability of a virus to infect host cells. By measuring the reduction in viral infectivity in the presence of specific antibodies, researchers can determine the neutralizing capability of these antibodies.

OUR PATHOGENS

We have a large panel of strains available and the ability to develop the assay for previous and emerging influenza strains.
  • Virus Family
  • Adenovirus
  • Chikungunya virus
  • Dengue virus, serotype 1
  • Dengue virus, serotype 2
  • Dengue virus, serotype 3
  • Dengue virus, serotype 4
  • Human cytomegalovirus
  • Herpes Simplex I Virus
  • Influenza A (H1N1)
  • Influenza A (H1N1) Antiviral Resistant (AVR)
  • Influenza A (H3N2) Antiviral Resistant (AVR)
  • Influenza B
  • Influenza B (Yamagata Lineage)
  • Influenza B (Victoria Lineage)
  • Respiratory Syncytial Virus A
  • Respiratory Syncytial Virus B
  • Zika virus

We offer an extensive range of viruses for your testing needs and can develop custom microneutralization assays to meet your specific research objectives.

Respiratory Viruses – Influenza, RSV, ADV

Arboviruses – Dengue 1-4, CHKV, JEV, LACV, RVF, YFV, ZIKV

Herpes Viruses – CMV, HSV-1, HSV-2, CMV

Enteric Viruses – CVA, Enteroviruses, Echoviruses

Pseudoviruses – Filoviruses, SARS-CoV-2

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These assays are invaluable tools for understanding the immune response to viral infections and the assessment of vaccines. They provide important information about the presence and potency of neutralizing antibodies, which are crucial for protection against many viral diseases.

Microneutralization Assays

Applications of Microneutralization Assays

Immunology Research

Microneutralization Assays are used to study the immune response to viral infections, vaccine efficacy, and the development of immunity in individuals or populations.

Vaccine Development

 These assays play a critical role in evaluating the effectiveness of vaccines by assessing their ability to induce neutralizing antibodies.

Epidemiology

MN assays are employed in seroepidemiological studies to estimate the prevalence of specific antibodies within a population, providing insights into the history and prevalence of viral infections.

Diagnostic Testing

Some MN assays are used for diagnostic purposes, especially for confirming the presence of antibodies to specific viruses, such as in the case of diagnosing certain viral diseases like measles or mumps.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL SUPPORT

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.