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Reliable HAI (Hemagglutination Inhibition Assay) services are essential for assessing the efficacy of vaccines and determining antibody levels against specific pathogens. At IBT Bioservices, we specialize in providing accurate and efficient HAI assay testing, offering comprehensive solutions to meet your research and development needs.

HAI ASSAY SERVICES

MOUSE SERUM TESTED AGAINST LIVE VIRUS (IFV-A/CA/07/2009)

Test articles (TAs) 1-5 are mouse serum samples collected post-vaccination. Serum is tested for antibodies against live virus IFV-A/CA/07/2009 in this hemagglutination inhibition (HAI) assay. Each TA is given a HAI titer, which is the highest dilution of serum to completely inhibit hemagglutination, indicated by a full teardrop of unagglutinated red blood cells. The HAI titers of TA1 –TA5 are 40, 320, 320, 80, and 160.

HUMAN SERUM TESTED AGAINST INACTIVATED VIRUS (IFV-A/VICTORIA/2570/2019)

TAs 1-4 are human serum samples collected pre- and post-vaccination. Serum is tested for antibodies against inactivated IFV-A/Victoria/2570/2019 in this hemagglutination inhibition (HAI) assay. Each TA is given a HAI titer, which is the highest dilution of serum to completely inhibit hemagglutination, indicated by a full teardrop of unagglutinated red blood cells. The HAI titers of TA1 –TA4 are 160, 320, 640, and 640. The positive control has an HAI titer of 640.

This assay tests for hemagglutinin (HA)-specific antibodies against influenza virus (INFV). Serial dilutions of the TA starting are incubated with a standardized amount of IFV antigen and Turkey Red Blood Cells (TRBCs) in 96-well plates. In the absence of antibodies against influenza hemagglutinin (HA) glycoprotein, the HA protein agglutinates TRBCs. Hemagglutination is inhibited in the presence of HA-specific antibodies, which will bind to the antigenic sites on the HA protein. The number of antibodies present in a sample is quantified as an HA titer, given as the highest dilution of serum that completely inhibits hemagglutination. IBT’s method is standardized to the WHO testing protocol and can use live virus for testing.

OUR PATHOGENS

We have a large panel of strains available and the ability to develop the assay for previous and emerging influenza strains.
  • A/New Caledonia/20/1999
  • A/Sydney/05/97
  • B/Florida/04/2006
  • B/Hong Kong/330/01
  • A/SolomonIslands/3/2006
  • A/Fujian/411/2002 (H3N2)
  • B/Wisconsin/1/2010
  • B/Brisbane/60/2008
  • A/Brisbane/59/2007
  • Respiratory Syncytial Virus B
  • A/Wisconsin/67/2005
  • B/Massachusetts/2/2012
  • B/Colorado/06/2017
  • A/California/7/2009
  • A/Brisbane/10/2007
  • B/Phuket/3073/2013
  • B/Washington/02/2019
  • A/Michigan/45/2015
  • A/Perth/16/2009
  • B/Phuket/3073/2013 (Yamagata Lineage)
  • B/Michigan/01/2021 (Victoria Lineage)
  • A/Wisconsin/588/2019
  • A/Texas/50/2012
  • B/Sichuan/379/99
  • B/Washington/02/2019 (Victoria Lineage)
  • A/Victoria/2570/2019
  • A/Switzerland/9715293/2013
  • B/Shanghai/361/2002
  • B/Austria/1359417/2021
  • A/Victoria/2570/2019 pdm09
  • A/Hong Kong/4801/2014
  • B/Phuket/3073/2013
  • B/Washington/02/2019 (egg-based)

We Have A Large Panel Of Strains Available And The Ability To Develop The Assay For Previous And Emerging Influenza Strains.

Influenza A/Puerto Rico/8/34 (H1N1)

Influenza A/California/07/2009 (H1N1)

Influenza A/Wisconsin/588/2019 (H1N1) pdm09

Influenza B/Phuket/3073/2013 (BY)

Influenza B/Phuket/3073/2013 (BY)

CONTACT US
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With years of experience in the field, our team of skilled scientists and technicians are well-versed in conducting HAI assays for a wide range of influenza strains. We utilize state-of-the-art equipment and follow stringent quality control measures to ensure precise and reproducible results.

OUR EXPERTISE

COMPREHENSIVE IN VITRO ASSAY SERVICES

CUSTOMIZED ASSAY DESIGN

We understand that every research project is unique. Our experts work closely with you to design customized HAI assays tailored to your specific requirements, ensuring optimal sensitivity and specificity.

SAMPLE ANALYSIS

Whether you need to analyze a large number of samples or perform a small-scale study, our laboratory is fully equipped to handle both high-throughput screening and low-volume testing. We offer quick turnaround times without compromising the quality and accuracy of the results.

QUALITY CONTROL

We adhere to strict quality control protocols to maintain the integrity of our assays. Our team conducts regular internal quality assessments, and our processes are designed to meet or exceed industry standards, ensuring reliable and reproducible data.

DATA ANALYSIS AND REPORTING

Our services extend beyond performing the assay. We provide comprehensive data analysis and interpretation to help you make informed decisions. Our reports are clear, concise, and include all the necessary details, allowing you to easily understand and present your findings.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL CUSTOMER SUPPORT

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.