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At IBT Bioservices, we offer reliable and comprehensive CellTiter-Glo assay services to assess cell viability, proliferation, and cytotoxicity. The CellTiter-Glo assay is a luminescent-based method that measures cellular ATP levels, providing valuable insights into cell health and response to various treatments.

CELL TITER-GLO ASSAY SERVICES

CYTOTOXICITY OF SMALL MOLECULES 1 AND 2 USING CELLTITER® GLO

Cytotoxicity data is graphed using luminescence in relative light units (RLU). Cells were incubated with small molecules 1 and 2 for a predetermined amount of time and Cell Titer Glo® was added to the cells to obtain RLU readings.

% CYTOTOXICITY OF SMALL MOLECULES 1 AND 2 USING CELLTITER® GLO

Small molecules 1 and 2 were tested for cytotoxicity in A549 cells. After a 72 hour incubation, small molecule 1 is toxic at most of the tested concentrations. Small molecule 2 is slightly toxic at the highest tested concentrations and nontoxic for the remaining tested concentrations.

Our experienced team of scientists utilizes optimized protocols and state-of-the-art equipment to ensure accurate and sensitive measurements in CellTiter-Glo assays. By quantifying ATP levels, we can precisely assess cell viability, proliferation, and cytotoxicity, even in low cell density or complex biological samples. We can identify potential cytotoxic or cytostatic effects, and determine CC50 values. These data can be used for compound screening and assist in the identification of promising drug candidates and guide further optimization.

OUR PATHOGENS

We have a large panel of strains available and the ability to develop the assay for previous and emerging influenza strains.

We can modify this assay to assess any of the following variables

Cell lines

Screening of small molecules, antibodies, and serum

Incubation times

Single concentration

Formualtion testing

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We adhere to strict quality control measures throughout the assay process. We maintain rigorous laboratory practices, adhere to standard operating procedures, and perform regular quality checks to ensure the reliability and accuracy of our results. Your research is in safe hands with our commitment to quality and precision.

STRICT QUALITY CONTROL

KEY FEATURES OF OUR CELLTITER-GLO ASSAY SERVICES

CUSTOMIZED ASSAY DESIGN AND OPTIMIZATION

We understand that each research study has unique requirements, and we tailor our CellTiter-Glo assays to meet your specific needs. Our scientists work closely with you to design and optimize the assay conditions, including cell seeding density, treatment duration, and assay readout time, to generate reliable and meaningful results.

MULTIPLEXING AND COMBINATION STUDIES

The flexibility of the CellTiter-Glo assay allows for multiplexing with other assays or combination studies. We can integrate CellTiter-Glo with other endpoint assays, such as apoptosis assays or cell cycle analysis, to provide a more comprehensive understanding of cellular responses. This approach enables a deeper exploration of the mechanisms underlying drug efficacy or toxicity. We also have the capacity to perform high-throughput CellTiter-Glo assays, making it suitable for screening large compound libraries or testing multiple conditions simultaneously. Our automated systems ensure precise and efficient handling of samples, reducing variability and enhancing the scalability of your research.

 

HIGH-THROUGHPUT CAPABILITY

We have the capacity to perform high-throughput CellTiter-Glo assays, making it suitable for screening large compound libraries or testing multiple conditions simultaneously. Our automated systems ensure precise and efficient handling of samples, reducing variability and enhancing the scalability of your research.

DATA ANALYSIS AND REPORTING

Our team of experts provides comprehensive data analysis and interpretation of the CellTiter-Glo assay results. We deliver detailed reports that include raw data, statistical analysis, and visual representations to facilitate data-driven decision-making. Our clear and concise reports help you understand and communicate your research findings effectively.

WHY CHOOSE IBT?

EXPERTISE

Our team of scientists and technicians are highly skilled and experienced in conducting HAI assays, ensuring accurate and reliable results.

CUSTOMIZED SOLUTIONS

We understand the unique needs of each research project and provide customized assay designs to meet your specific requirements.

TIMELY RESULTS

We prioritize efficiency without compromising on quality. Our streamlined processes and advanced equipment allow for quick turnaround times, ensuring you receive results promptly.

QUALITY ASSURANCE

We maintain rigorous quality control measures throughout our operations, ensuring the accuracy and reproducibility of our assay results.

EXCEPTIONAL SUPPORT

Our dedicated customer support team is always available to address your queries and provide assistance at every step of the process.