Cytokine and Biomarker Analysis

Using a highly sensitive electrochemiluminesence technology developed by Meso Scale Discovery™, IBT Bioservices offers custom services for testing cytokines, chemokines and other biomarkers in culture supernatants and sera.  Current service offerings include:

Panel Species Analyte
Th1/Th2 (10-Plex) Human IFNg, IL1b, IL-2, IL-4, IL-5, IL-8, IL-10, IL-12, IL-13, TNF-a
Th1/Th2 (9-Plex) Mouse IFNg, IL1b, IL-2, IL-4, IL-5, KC/GRO/CINC, IL-10, IL-12, TNF-a
Pro-inflammatory I (4-Plex) Human IFNg, IL1b, IL-6, TNF-a
Pro-inflammatory II (4-Plex) Human IL1b, IL-6, IL-8, TNF-a
Chemokine (9-Plex) Human Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1b, TARC

Meso Scale Discovery™ (MSD) based assays:

MSD is a revolutionary technology that utilizes electrochemiluminescence detection to detect binding events on patterned arrays. The combination forms a unique solution offering sensitivity, dynamic range and convenience. MSD based assays have a wide dynamic range typically from 10ˆ3 to 10ˆ4 allowing quantitative measurements with high accuracy and without the need for running multiple dilutions. MSD assays can be also established in a multiplex format with 5-10 antigens per well.

Unique Advantages of MSD

  • Capture: 10-100 fold increase in capture capacity compared to ELISA
  • Dynamic Range: 3-4 log dynamic range vs. 1-1.5 log range for ELISA
  • Sensitivity: Detection of analytes at sub-picogram levels
  • Reduced Sample Volume: 5-25 ul volume vs. 50-100 ul for ELISA
  • Speed: The entire assay can be performed in 3 hours
  • Multiplex Capability: Simultaneous measurements for up to 10 analytes


**Note: The data above is not a head to head experiment and is provided only to illustrate the advantage of increased dynamic range with MSD over ELISA.

Contact us today:

Email: services@ibtbioservices.com

Toll Free Phone: (877) 411-2041

Fax: (240) 778-6805