Antibiotic Testing
Detection of In vitro Antibiotic Activity
Minimum Inhibitory Concentration (MIC) Assay – The MIC assay is a technique used to determine the lowest concentration of a particular antimicrobial needed to inhibit visible growth of bacteria. This assay is typically performed on planktonic (free floating) bacterial cells. MIC is determined by broth microdilution followed by inoculation onto agar plates for enumeration.
Minimal bactericidal concentration (MBC) - The MBC is the lowest concentration of antimicrobial required to kill an organism. To determine the MBC, cultures are incubated with serial dilutions of the compound(s) of interest, inoculated onto agar plates and enumerated. A colony count that represents 0.1% (99.9% reduction) of the original inoculum is considered as MBC.
Minimal doubling time/growth curve (MDT) – Additional testing could include the performance of a MDT of the strains in the presence or absence of the compound.
Bacteria currently available for testing (additional bacterial species can be acquired for purposes of individual interests):
| Bacterial species | Number of isolates available | Details |
| Methicillin-SensitiveStaphylococcus aureus | 19 | Clinical and standard laboratory strains including 1 isogenic couple |
| Methicillin-ResistantStaphylococcus aureus | 10 | Clinical and standard laboratory strains including 2 isogenic couples |
| Pseudomonas aeruginosa | 12 | Clinical strains |
| Acinetobacter baumannii | 20 | Clinical strains |
| Enterobacter aerogenes | 1 | Clinical strains |
| Enterobacter cloacae | 6 | Clinical strains including ESBL, MDR, AMPC |
| Streptococcus pneumoniae | 4 | Clinical strains |
| Moraxella catarrhalis | 3 | Clinical strains |
Contact us today:
Email: services@ibtbioservices.com
Toll Free Phone: (877) 411-2041
Fax: (240) 778-6805